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multiscripbe reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscripbe reverse transcriptase
    Multiscripbe Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscripbe+reverse+transcriptase/pmc06927743-245-26-29
    Average 90 stars, based on 1 article reviews
    multiscripbe reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Article Snippet: Cultured neurons were lysed using 50 mM Tris/HCl buffer (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10 mM sodium pyrophosphate, 20 mM NaF, 1 mM sodium orthovanadate and 1% complete protease inhibitor cocktail (Roche) at 4°C for 20 min. Co-immunoprecipitation and western blot analysis were performed as previously described ( ). .. Total RNA was isolated from hippocampal neuron cultures with TRIzol reagent (Thermo Fisher) according to the manufacturer’s protocol. cDNA was generated by reverse transcription PCR using MultiScripbe reverse transcriptase (Thermo Fisher) with random primers. .. Synthesized cDNA was used for quantitative real-time PCR with the TaqMan System (Thermo Fisher) and the following primers were used: beta-actin FAM (Mm00607939_s1, FAM, Applied Biosystems), VEGFR2 (Mm00440085_m1, FAM, Applied Biosystems).

    Article Title: EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Article Snippet: Co-Immunoprecipitation and western blot analysis Cultured neurons were lysed using 50 mM Tris/HCl buffer (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10 mM sodium pyrophosphate, 20 mM NaF, 1 mM sodium orthovanadate and 1% complete protease inhibitor cocktail (Roche) at 4 ̊C for 20 min. Co-immunoprecipitation and western blot analysis were performed as previously described (Essmann et al., 2008). .. Total RNA was isolated from hippocampal neuron cultures with TRIzol reagent (Thermo Fisher) according to the manufacturer’s protocol. cDNA was generated by reverse transcription PCR using MultiScripbe reverse transcriptase (Thermo Fisher) with random primers. .. Synthesized cDNA was used for quantitative real-time PCR with the TaqMan System (Thermo Fisher) and the following primers were used: beta-actin FAM (Mm00607939_s1, FAM, Applied Biosystems), VEGFR2 (Mm00440085_m1, FAM, Applied Biosystems).

    Generated:

    Article Title: EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Article Snippet: Cultured neurons were lysed using 50 mM Tris/HCl buffer (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10 mM sodium pyrophosphate, 20 mM NaF, 1 mM sodium orthovanadate and 1% complete protease inhibitor cocktail (Roche) at 4°C for 20 min. Co-immunoprecipitation and western blot analysis were performed as previously described ( ). .. Total RNA was isolated from hippocampal neuron cultures with TRIzol reagent (Thermo Fisher) according to the manufacturer’s protocol. cDNA was generated by reverse transcription PCR using MultiScripbe reverse transcriptase (Thermo Fisher) with random primers. .. Synthesized cDNA was used for quantitative real-time PCR with the TaqMan System (Thermo Fisher) and the following primers were used: beta-actin FAM (Mm00607939_s1, FAM, Applied Biosystems), VEGFR2 (Mm00440085_m1, FAM, Applied Biosystems).

    Article Title: EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Article Snippet: Co-Immunoprecipitation and western blot analysis Cultured neurons were lysed using 50 mM Tris/HCl buffer (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10 mM sodium pyrophosphate, 20 mM NaF, 1 mM sodium orthovanadate and 1% complete protease inhibitor cocktail (Roche) at 4 ̊C for 20 min. Co-immunoprecipitation and western blot analysis were performed as previously described (Essmann et al., 2008). .. Total RNA was isolated from hippocampal neuron cultures with TRIzol reagent (Thermo Fisher) according to the manufacturer’s protocol. cDNA was generated by reverse transcription PCR using MultiScripbe reverse transcriptase (Thermo Fisher) with random primers. .. Synthesized cDNA was used for quantitative real-time PCR with the TaqMan System (Thermo Fisher) and the following primers were used: beta-actin FAM (Mm00607939_s1, FAM, Applied Biosystems), VEGFR2 (Mm00440085_m1, FAM, Applied Biosystems).

    Reverse Transcription:

    Article Title: EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Article Snippet: Cultured neurons were lysed using 50 mM Tris/HCl buffer (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10 mM sodium pyrophosphate, 20 mM NaF, 1 mM sodium orthovanadate and 1% complete protease inhibitor cocktail (Roche) at 4°C for 20 min. Co-immunoprecipitation and western blot analysis were performed as previously described ( ). .. Total RNA was isolated from hippocampal neuron cultures with TRIzol reagent (Thermo Fisher) according to the manufacturer’s protocol. cDNA was generated by reverse transcription PCR using MultiScripbe reverse transcriptase (Thermo Fisher) with random primers. .. Synthesized cDNA was used for quantitative real-time PCR with the TaqMan System (Thermo Fisher) and the following primers were used: beta-actin FAM (Mm00607939_s1, FAM, Applied Biosystems), VEGFR2 (Mm00440085_m1, FAM, Applied Biosystems).

    Article Title: EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Article Snippet: Co-Immunoprecipitation and western blot analysis Cultured neurons were lysed using 50 mM Tris/HCl buffer (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10 mM sodium pyrophosphate, 20 mM NaF, 1 mM sodium orthovanadate and 1% complete protease inhibitor cocktail (Roche) at 4 ̊C for 20 min. Co-immunoprecipitation and western blot analysis were performed as previously described (Essmann et al., 2008). .. Total RNA was isolated from hippocampal neuron cultures with TRIzol reagent (Thermo Fisher) according to the manufacturer’s protocol. cDNA was generated by reverse transcription PCR using MultiScripbe reverse transcriptase (Thermo Fisher) with random primers. .. Synthesized cDNA was used for quantitative real-time PCR with the TaqMan System (Thermo Fisher) and the following primers were used: beta-actin FAM (Mm00607939_s1, FAM, Applied Biosystems), VEGFR2 (Mm00440085_m1, FAM, Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Article Snippet: Cultured neurons were lysed using 50 mM Tris/HCl buffer (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10 mM sodium pyrophosphate, 20 mM NaF, 1 mM sodium orthovanadate and 1% complete protease inhibitor cocktail (Roche) at 4°C for 20 min. Co-immunoprecipitation and western blot analysis were performed as previously described ( ). .. Total RNA was isolated from hippocampal neuron cultures with TRIzol reagent (Thermo Fisher) according to the manufacturer’s protocol. cDNA was generated by reverse transcription PCR using MultiScripbe reverse transcriptase (Thermo Fisher) with random primers. .. Synthesized cDNA was used for quantitative real-time PCR with the TaqMan System (Thermo Fisher) and the following primers were used: beta-actin FAM (Mm00607939_s1, FAM, Applied Biosystems), VEGFR2 (Mm00440085_m1, FAM, Applied Biosystems).

    Article Title: EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Article Snippet: Co-Immunoprecipitation and western blot analysis Cultured neurons were lysed using 50 mM Tris/HCl buffer (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10 mM sodium pyrophosphate, 20 mM NaF, 1 mM sodium orthovanadate and 1% complete protease inhibitor cocktail (Roche) at 4 ̊C for 20 min. Co-immunoprecipitation and western blot analysis were performed as previously described (Essmann et al., 2008). .. Total RNA was isolated from hippocampal neuron cultures with TRIzol reagent (Thermo Fisher) according to the manufacturer’s protocol. cDNA was generated by reverse transcription PCR using MultiScripbe reverse transcriptase (Thermo Fisher) with random primers. .. Synthesized cDNA was used for quantitative real-time PCR with the TaqMan System (Thermo Fisher) and the following primers were used: beta-actin FAM (Mm00607939_s1, FAM, Applied Biosystems), VEGFR2 (Mm00440085_m1, FAM, Applied Biosystems).



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    90
    Thermo Fisher multiscripbe reverse transcriptase
    Multiscripbe Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscripbe+reverse+transcriptase/pmc06927743-245-26-29
    Average 90 stars, based on 1 article reviews
    multiscripbe reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars
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